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Inhibitory effect of MKE on the TLR4-NFκB signaling pathway. The expression levels of <t>TLR-4,</t> p-IκBα/IκBα, and p-p65/p65 in tissues collected at week 16 were evaluated. ( A ) Representative Western blot image. ( B ) Quantitative analysis of TLR-4 expression. ( C ) Quantitative analysis of the p-IκBα/IκBα expression ratio. ( D ) Quantitative analysis of the p-p65/p65 ratio. The data are expressed as mean ± standard deviation ( n = 3/group). * p < 0.05, ** p < 0.01 compared to the ligature control group; # p < 0.05 versus the non-ligature control group. Non-ligature = non-ligature control group, Ligature = ligature control group, Doxycycline = ligature + doxycycline 20 mg/kg group, MKE 100 = ligature + MKE 100 mg/kg group, MKE 400 = ligature + MKE 400 mg/kg group.
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Inhibitory effect of MKE on the TLR4-NFκB signaling pathway. The expression levels of TLR-4, p-IκBα/IκBα, and p-p65/p65 in tissues collected at week 16 were evaluated. ( A ) Representative Western blot image. ( B ) Quantitative analysis of TLR-4 expression. ( C ) Quantitative analysis of the p-IκBα/IκBα expression ratio. ( D ) Quantitative analysis of the p-p65/p65 ratio. The data are expressed as mean ± standard deviation ( n = 3/group). * p < 0.05, ** p < 0.01 compared to the ligature control group; # p < 0.05 versus the non-ligature control group. Non-ligature = non-ligature control group, Ligature = ligature control group, Doxycycline = ligature + doxycycline 20 mg/kg group, MKE 100 = ligature + MKE 100 mg/kg group, MKE 400 = ligature + MKE 400 mg/kg group.

Journal: Current Issues in Molecular Biology

Article Title: Protective Effects of Magnolia kobus DC. Extract on Inflammatory Response and Alveolar Bone Loss in Ligature-Induced Periodontitis Rats

doi: 10.3390/cimb48010109

Figure Lengend Snippet: Inhibitory effect of MKE on the TLR4-NFκB signaling pathway. The expression levels of TLR-4, p-IκBα/IκBα, and p-p65/p65 in tissues collected at week 16 were evaluated. ( A ) Representative Western blot image. ( B ) Quantitative analysis of TLR-4 expression. ( C ) Quantitative analysis of the p-IκBα/IκBα expression ratio. ( D ) Quantitative analysis of the p-p65/p65 ratio. The data are expressed as mean ± standard deviation ( n = 3/group). * p < 0.05, ** p < 0.01 compared to the ligature control group; # p < 0.05 versus the non-ligature control group. Non-ligature = non-ligature control group, Ligature = ligature control group, Doxycycline = ligature + doxycycline 20 mg/kg group, MKE 100 = ligature + MKE 100 mg/kg group, MKE 400 = ligature + MKE 400 mg/kg group.

Article Snippet: Toll-like receptor 4 (TLR-4) , Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Standard Deviation, Control

The postulated mechanism underlying the anti-periodontitis activity of MKE. MKE = Magnolia kobus DC. extract, ROS = reactive oxygen species, TLR-4 = toll-like receptor 4, p-IκBα = phosphorylated inhibitor of κBα, IκBα = inhibitor of κBα, IL-1β = interleukin-1β, IL-6 = interleukin-6, TNF-α = tumor necrosis factor-α, COX-2 = cyclooxygenase-2, MMPs = matrix metalloproteinases.

Journal: Current Issues in Molecular Biology

Article Title: Protective Effects of Magnolia kobus DC. Extract on Inflammatory Response and Alveolar Bone Loss in Ligature-Induced Periodontitis Rats

doi: 10.3390/cimb48010109

Figure Lengend Snippet: The postulated mechanism underlying the anti-periodontitis activity of MKE. MKE = Magnolia kobus DC. extract, ROS = reactive oxygen species, TLR-4 = toll-like receptor 4, p-IκBα = phosphorylated inhibitor of κBα, IκBα = inhibitor of κBα, IL-1β = interleukin-1β, IL-6 = interleukin-6, TNF-α = tumor necrosis factor-α, COX-2 = cyclooxygenase-2, MMPs = matrix metalloproteinases.

Article Snippet: Toll-like receptor 4 (TLR-4) , Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activity Assay